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Ghost! question about Ca2+ in embryo culture

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Ghost! question about Ca2+ in embryo culture

Postby leorira11 » Wed Mar 20, 2013 4:22 am

I've been looking for information on this and can't find it anywhere - maybe you can help?

I've done 7 fresh IVF cycles and 4 FETs (mostly eSET). I've been pregnant 5 times and have no living children (2 first trimester miscarriages, a missed miscarriage at 18 weeks, two sets of twins born prematurely and died).

About halfway through, I switched clinics. At my new clinic, the supposedly 'better' lab is getting worse results and no one knows why. In the four cycles I've had there:

1 - of 17 eggs, only one fertilized = BFN
2- of 12 eggs - only one made it to day 5 = baby boy lost at 18 weeks
3 - of 11 eggs - only 2 fertilized = BFN
4- of 21 eggs - seven made it to day 5, transferred one (and the rest weren't good enough to freeze...) = pending...

Our fertility issue is severe male factor (using ICSI). I'm 26, my husband is 28.

At my old clinic, we always had 2-3 embryos on day 5 (out of a similar number of eggs 12-18 each time)

I got a second opinion from a doctor who ended up being a crackpot. He looked at the embryology reports and saw that there is a marking that they added Ca2+ to the culture. He said they only do that when there is a problem with the eggs and suggested we try donor eggs.

My regular RE says that's nonsense, but he doesn't know why they added Ca2+ to the culture.

I've been trying to research on my own and can't find anything!

any help? Does Ca2+ added to culture mean that there is a problem with egg quality?

Thanks!

Leora
8 IVF+6 FET=6 BFN+8 BFP =

-b/g twins 22w (12.09)
-mc 10w (9.10)
-Micha (7.19-24.11) & Asaf (7.19-28.11) born at 24w
-mc 5wk (2.12)
-no HB at 18w (10.12)
-BO (4.13)-
-mc 6wk (9.13)

last attempt - donor sperm - baby girl born healthy July 2014
leorira11
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Posts: 1275
Joined: Fri Mar 19, 2010 12:09 pm
Location: Jerusalem, Israel

Re: Ghost! question about Ca2+ in embryo culture

Postby Ghost » Sat Mar 23, 2013 1:28 am

leorira11 wrote:My regular RE says that's nonsense, but he doesn't know why they added Ca2+ to the culture.


I tend to agree. We don't really know why they altered the media.

If your problem is male factor, why is the solution donor egg???
Avoid IVF and surrogacy in Ukraine. Ukrainian centers pay shills to post here under numerous sock accounts pretending to be patients in Ukraine. Centers using such deceptive advertising cannot be trusted and should be avoided.
Ghost
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Posts: 4151
Joined: Mon May 29, 2006 5:01 pm

Re: Ghost! question about Ca2+ in embryo culture

Postby leorira11 » Sat Mar 23, 2013 8:18 am

well, our reason for doing IVF is male factor, but we have horrific fertilization rates. This crack-pot doctor says that ICSI solves all that, so our fertilization rate issue is NOT our male factor - and is either a lab issue (not likely, since I'm at the best lab in the country) or an egg issue - hence the donor egg suggestion.

we tried a whole bunch of new things on our last cycle-- long lupron (I'd always done antagonist before), I started taking Metformin (I'm PCO), we tried IMSI

8dp5dt.... BFP!

Overall, out of 16 embryos transferred (some of which were crappy quality) - 8 have gotten me pregnant -- so we seem to be doing okay with the IVF.

Hopefully this one will stick and be the baby we get to bring home with us!
8 IVF+6 FET=6 BFN+8 BFP =

-b/g twins 22w (12.09)
-mc 10w (9.10)
-Micha (7.19-24.11) & Asaf (7.19-28.11) born at 24w
-mc 5wk (2.12)
-no HB at 18w (10.12)
-BO (4.13)-
-mc 6wk (9.13)

last attempt - donor sperm - baby girl born healthy July 2014
leorira11
Valued Contributor
 
Posts: 1275
Joined: Fri Mar 19, 2010 12:09 pm
Location: Jerusalem, Israel

Re: Ghost! question about Ca2+ in embryo culture

Postby leorira11 » Sat Mar 23, 2013 8:18 am

well, our reason for doing IVF is male factor, but we have horrific fertilization rates. This crack-pot doctor says that ICSI solves all that, so our fertilization rate issue is NOT our male factor - and is either a lab issue (not likely, since I'm at the best lab in the country) or an egg issue - hence the donor egg suggestion.

we tried a whole bunch of new things on our last cycle-- long lupron (I'd always done antagonist before), I started taking Metformin (I'm PCO), we tried IMSI

8dp5dt.... BFP!

Overall, out of 16 embryos transferred (some of which were crappy quality) - 8 have gotten me pregnant -- so we seem to be doing okay with the IVF.

Hopefully this one will stick and be the baby we get to bring home with us!
8 IVF+6 FET=6 BFN+8 BFP =

-b/g twins 22w (12.09)
-mc 10w (9.10)
-Micha (7.19-24.11) & Asaf (7.19-28.11) born at 24w
-mc 5wk (2.12)
-no HB at 18w (10.12)
-BO (4.13)-
-mc 6wk (9.13)

last attempt - donor sperm - baby girl born healthy July 2014
leorira11
Valued Contributor
 
Posts: 1275
Joined: Fri Mar 19, 2010 12:09 pm
Location: Jerusalem, Israel


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